Student: Sasha Smerekanych
Mentor: Irimpan Mathews
Project Title: Structural study of a receptor involved in cell proliferation
Project Description: Granulocyte colony stimulating factor (G-CSF) is a hematopoietic cytokine that stimulates neutrophil production and hematopoietic stem cell mobilization by initiating the dimerization of homodimeric granulocyte colony stimulating factor receptor (G-CSFR). Different mutations of CSF3R have been linked to a unique spectrum of myeloid disorders and related malignancies. Mutations in the CSF3R have recently been found in a large percentage of patients with chronic neutrophilic leukemia and, more rarely, in other types of leukemia. The current project involves functional study of the protein related to CSF3R. Various proteins are currently under expression and purification. We will use X-ray crystallography as the main tool to get molecular level understanding of the receptor. Students will learn various aspects of X-ray crystallography including crystallization, data collection and structure solution.